Rapid and efficient identification of the mouse leptin receptor mutation (C57BL/KsJ-db/db) by tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) analysis

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Rapid and efficient identification of the mouse leptin receptor mutation (C57BL/KsJ-db/db) by tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) analysis

The C57BLKS/J-Lepr(db) mouse has a point mutation in the leptin receptor gene and is one of the most useful animal model for non-insulin dependent diabetes mellitus in human. Since the homozygote of C57BLKS/J-Lepr(db) mouse is infertile, detection of point mutation in the leptin receptor gene is important for efficient maintaining strains as well as mass production of homozygotes. To develop a ...

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multiplex tetra-primer amplification refractory mutation system polymerase chain reaction to genotype snp8nrg221533 of neuregulin-1 gene

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ژورنال

عنوان ژورنال: Laboratory Animal Research

سال: 2016

ISSN: 1738-6055,2233-7660

DOI: 10.5625/lar.2016.32.1.70